| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Spastin (163-213 aa) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPAST |
| Gene ID: | 6683 |
| Uniprot ID: | SPAST_HUMAN |
| Immunogen Region: | 163-213 aa |
| Specificity: | This antibody detects endogenous levels of SPAST at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human SPAST at the amino acid range 163-213 |
| Function | ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Severing activity is not dependent on tubulin acetylation or detyrosination. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex. Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex. Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling. Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing. Probably plays a role in axon growth and the formation of axonal branches. Isoform 1: Involved in lipid metabolism by regulating the size and distribution of lipid droplets. |
| Protein Name | Spastin Spastic Paraplegia 4 Protein |
| Database Links | Reactome: R-HSA-9668328 |
| Cellular Localisation | Membrane Peripheral Membrane Protein Endoplasmic Reticulum Midbody Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Perinuclear Region Nucleus Spindle Cell Projection Axon Forms An Intramembrane Hairpin-Like Structure In The Membrane Localization To The Centrosome Is Independent Of Microtubules Localizes To The Midbody Of Dividing Cells And This Requires Chmp1b Enriched In The Distal Axons And Branches Of Postmitotic Neurons Mainly Nuclear In Interphase Cells And Becomes Associated With The Centrosomes Spindle Microtubules Midzone And Finally The Midbody During Cell Division Isoform 1: Endoplasmic Reticulum Membrane Nucleus Membrane Lipid Droplet Endosome Recruited To Nuclear Membrane By Ist1 During Late Anaphase Localizes To Endoplasmic Reticulum Tubular Network Isoform 3: Cytoplasm Constitutes The Main Endosomal Form |
| Alternative Antibody Names | Anti-Spastin antibody Anti-Spastic Paraplegia 4 Protein antibody Anti-SPAST antibody Anti-ADPSP antibody Anti-FSP2 antibody Anti-KIAA1083 antibody Anti-SPG4 antibody |
Information sourced from Uniprot.org