• Western blot analysis of lysates from Rat brain, using SPARCL1 Rabbit polyclonal antibody (STJ11103442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of lysates from Mouse brain, using SPARCL1 Rabbit polyclonal antibody (STJ11103442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPARCL1 antibody (17-300) (STJ11103442)

SKU:
STJ11103442

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPARCL1
Gene ID: 8404
Uniprot ID: SPRL1_HUMAN
Immunogen Region: 17-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 17-300 of human SPARCL1 (NP_004675.3).
Immunogen Sequence: IPTNARLLSDHSKPTAETVA PDNTAIPSLRAEAEENEKET AVSTEDDSHHKAEKSSVLKS KEESHEQSAEQGKSSSQELG LKDQEDSDGHLSVNLEYAPT EGTLDIKEDMSEPQEKKLSE NTDFLAPGVSSFTDSNQQES ITKREENQEQPRNYSHHQLN RSSKHSQGLRDQGNQEQDPN ISNGEEEEEKEPGEVGTHND NQERKTELPREHANSKQEED NTQSDDILEESDQPTQVSK
Tissue Specificity Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
Post Translational Modifications N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Protein Name Sparc-Like Protein 1
High Endothelial Venule Protein
Hevin
Mast 9
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Sparc-Like Protein 1 antibody
Anti-High Endothelial Venule Protein antibody
Anti-Hevin antibody
Anti-Mast 9 antibody
Anti-SPARCL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance