| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SP3 (705-781) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SP3 |
| Gene ID: |
6670 |
| Uniprot ID: |
SP3_HUMAN |
| Immunogen Region: |
705-781 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 705-781 of human SP3 (NP_003102.1). |
| Immunogen Sequence: |
NKKGIHSSSTVLASVEAARD DTLITAGGTTLILANIQQGS VSGIGTVNTSATSNQDILTN TEIPLQLVTVSGNETME |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Not glycosylated. Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter. Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. |
| Function | Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping. |
| Protein Name | Transcription Factor Sp3Spr-2 |
| Database Links | Reactome: R-HSA-3232118 |
| Cellular Localisation | NucleusPml BodyLocalizes To The Nuclear Periphery And In Nuclear Dots When SumoylatedSome Localization In Pml Nuclear Bodies |
| Alternative Antibody Names | Anti-Transcription Factor Sp3 antibodyAnti-Spr-2 antibodyAnti-SP3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
