• STJ73741 (0. 3µg/ml) staining of MCF7 nuclear (A) and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73741 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73741 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SOX2 antibody (N-Term) (STJ73741)

SKU:
STJ73741-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/FC/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SOX2 (N-Term) is suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IF-Strong expression of the protein seen in the nuclei of U251 cells. 10µg/ml
FC-Flow cytometric analysis of MCF7 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SOX2
Gene ID: 6657
Uniprot ID: SOX2_HUMAN
Immunogen Region: N-Term
Accession Number: NP_003097.1
Immunogen Sequence: YNMMETELKPPGPQQ
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development.
Protein Name Transcription Factor Sox-2
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-3769402
Reactome: R-HSA-452723
Reactome: R-HSA-6785807
Reactome: R-HSA-8986944
Reactome: R-HSA-9754189
Cellular Localisation Nucleus Speckle
Cytoplasm
Nucleus
Acetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic Delocalization
Colocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)
Colocalizes With Sox6 In Speckles
Colocalizes With Caml In The Nucleus
Nuclear Import Is Facilitated By Xpo4
A Protein That Usually Acts As A Nuclear Export Signal Receptor
Alternative Antibody Names Anti-Transcription Factor Sox-2 antibody
Anti-SOX2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance