| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SOX2 (10-70 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Sox2 |
| Gene ID: |
20674 |
| Uniprot ID: |
SOX2_MOUSE |
| Immunogen Region: |
10-70 aa |
| Specificity: |
Specific for SOX2. |
| Immunogen: |
A synthetic peptide from aa region of 10-70 of mouse SOX2 conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. Methylation at Lys-44 and Lys-119 is necessary for the regulation of SOX2 proteasomal degradation. Ubiquitinated by WWP2, leading to proteasomal degradation. |
| Function | Transcription factor that forms a trimeric complex with POU5F1 (OCT3/4) on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development. |
| Protein Name | Transcription Factor Sox-2 |
| Database Links | Reactome: R-MMU-3769402 |
| Cellular Localisation | NucleusCytoplasmNuclear Import Is Facilitated By Xpo4A Protein That Usually Acts As A Nuclear Export Signal Receptor |
| Alternative Antibody Names | Anti-Transcription Factor Sox-2 antibodyAnti-Sox2 antibodyAnti-Sox-2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
