• Immunohistochemistry analysis of paraffin-embedded mouse embryos using SOX1 Rabbit polyclonal antibody (STJ25659) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SOX1 antibody (STJ25659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SOX1 antibody (292-391) (STJ25659)

SKU:
STJ25659

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SOX1 (292-391) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOX1
Gene ID: 6656
Uniprot ID: SOX1_HUMAN
Immunogen Region: 292-391
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 292-391 of human SOX1 (NP_005977.2).
Immunogen Sequence: HQNSAVAAAAAAAAASSGAL GALGSLVKSEPSGSPPAPAH SRAPCPGDLREMISMYLPAG EGGDPAAAAAAAAQSRLHSL PQHYQGAGAGVNGTVPLTHI
Tissue Specificity Mainly expressed in the developing central nervous system.
Function Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation.
Protein Name Transcription Factor Sox-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Sox-1 antibody
Anti-SOX1 antibody

Information sourced from Uniprot.org

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