• STJ70903 (0. 02µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70903 (0. 01µg/ml) staining of NIH3T3 (A) , HEK293 (B) , HepG2 (C) and MCF7 (D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70903 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70903 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SOD1 antibody (Internal) (STJ70903)

SKU:
STJ70903-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SOD1 (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:8000.
WB: Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calcula
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SOD1
Gene ID: 6647
Uniprot ID: SODC_HUMAN
Immunogen Region: Internal
Accession Number: NP_000445.1
Immunogen Sequence: SRKHGGPKDEERH
Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Protein Name Superoxide Dismutase Cu-Zn
Superoxide Dismutase 1
Hsod1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-3299685
Reactome: R-HSA-8950505
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
The Pathogenic Variants Als1 Arg-86 And Ala-94 Gradually Aggregates And Accumulates In Mitochondria
Alternative Antibody Names Anti-Superoxide Dismutase Cu-Zn antibody
Anti-Superoxide Dismutase 1 antibody
Anti-Hsod1 antibody
Anti-SOD1 antibody

Information sourced from Uniprot.org

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