Biotinylated STJ70482 (2µg/ml) staining of A549 lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-SNX5, Biotinylated antibody (C-Term) {Biotin} (STJ73286)

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STJ73286-100
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Covered by St John's Laboratory Guarantee

Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-SNX5, Biotinylated (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 48kDa band observed in lysates of cell line A549 (calculated MW of 46.8kDa according to NP_055241.1). See non-biotinylated parental product's datasheet for further QC data. Reco
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SNX5
Gene ID: 27131
Uniprot ID: SNX5_HUMAN
Immunogen Region: C-Term
Accession Number: NP_055241.1; NP_001269383.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_055241.1; NP_001269383.1). Reported variants represent identical protein: NP_055241.1, NP_689413.1
Immunogen Sequence: SLLQSCIDLFKNN.
Protein Name Sorting Nexin-5
Database Links Reactome: R-HSA-432722
Cellular Localisation Endosome
Early Endosome
Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Membrane
Cytoplasmic Vesicle Membrane
Cytoplasm
Cell Projection
Phagocytic Cup
Ruffle
Recruited To The Plasma Membrane After Egf Stimulation
Which Leads To Increased Levels Of Phosphatidylinositol 3
4-Bisphosphate (Pdtins(3
4)P2)
Detected On Macropinosomes
Targeted To Membrane Ruffles In Response To Egfr Stimulation
Alternative Antibody Names Anti-Sorting Nexin-5 antibody
Anti-SNX5 antibody

Information sourced from Uniprot.org

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