Anti-SMYD4 antibody (1-260) (STJ116177)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SMYD4 (1-260) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMYD4 |
| Gene ID | 114826 |
| Uniprot ID | SMYD4_HUMAN |
| Immunogen Region | 1-260 |
| Immunogen Sequence | MDLPVDEWKSYLLQKWASLP TSVQVTISTAETLRDIFLHS SSLLQPEDELFLKRLSKGYL VGKDSDAPLFYREEGNKKFQ EKDYTGAAVLYSKGVSHSRP NTEDMSLCHANRSAALFHLG QYETCLKDINRAQTHGYPER LQPKIMLRKAECLVALGRLQ EASQTISDLERNFTATPALA DVLPQTLQRNLHRLKMKMQE KDSLTESFPAALAKTLEDAA LREENEQLSNASSSIGLCV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2). |
Additional Info
| Function | Protein-lysine N-methyltransferase. Monomethylates PRMT5, modulating its transcriptional activity. May also act as a histone methyltransferase. Plays a critical role in cardiac development. Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1. |
| Protein Name | Protein-Lysine N-Methyltransferase Smyd4Set And Mynd Domain-Containing Protein 4 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Protein-Lysine N-Methyltransferase Smyd4 antibodyAnti-Set And Mynd Domain-Containing Protein 4 antibodyAnti-SMYD4 antibodyAnti-KIAA1936 antibody |
Information sourced from Uniprot.org