• Immunofluorescence analysis of L929 cells using SMYD3 Rabbit polyclonal antibody (STJ116727) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SMYD3 Rabbit polyclonal antibody (STJ116727) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SMYD3 Rabbit polyclonal antibody (STJ116727) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SMYD3 in paraffin-embedded Human colon using SMYD3 Rabbit polyclonal antibody (STJ116727) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SMYD3 Rabbit polyclonal antibody (STJ116727) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMYD3 antibody (50-150) (STJ116727)

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STJ116727

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMYD3 (50-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMYD3
Gene ID: 64754
Uniprot ID: SMYD3_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SMYD3 (NP_001161212.1).
Immunogen Sequence: DRCLLGKEKLMRCSQCRVAK YCSAKCQKKAWPDHKRECKC LKSCKPRYPPDSVRLLGRVV FKLMDGAPSESEKLYSFYDL ESNINKLTEDKKEGLRQLVM T
Tissue Specificity Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
Function Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Protein Name Histone-Lysine N-Methyltransferase Smyd3
Set And Mynd Domain-Containing Protein 3
Zinc Finger Mynd Domain-Containing Protein 1
Database Links Reactome: R-HSA-3214841
Cellular Localisation Cytoplasm
Nucleus
Mainly Cytoplasmic When Cells Are Arrested At G0/G1
Accumulates In The Nucleus At S Phase And G2/M
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Smyd3 antibody
Anti-Set And Mynd Domain-Containing Protein 3 antibody
Anti-Zinc Finger Mynd Domain-Containing Protein 1 antibody
Anti-SMYD3 antibody
Anti-ZMYND1 antibody
Anti-ZNFN3A1 antibody

Information sourced from Uniprot.org

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