| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase SMURF2 (505-555 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMURF2 |
| Gene ID: | 64750 |
| Uniprot ID: | SMUF2_HUMAN |
| Immunogen Region: | 505-555 aa |
| Specificity: | This antibody detects endogenous levels of SMUF2 at Human/Mouse |
| Immunogen: | Synthesized peptide derived from the human SMUF2 at the amino acid range 505-555 |
| Post Translational Modifications | Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. Ubiquitinated by the SCF(FBXL15) complex and TTC3, leading to its degradation by the proteasome. 'Lys-48'-linked polyubiquitination mediated by TRAF4 at Lys-119 leads to SMURF2 proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1. Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation. SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation. Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation. (Microbial infection) In case of filoviruses Ebola/EBOV and Marburg/MARV infection, the complex formed by viral matrix protein VP40 and SMURF2 facilitates virus budding. |
| Protein Name | E3 Ubiquitin-Protein Ligase Smurf2 Hsmurf2 Hect-Type E3 Ubiquitin Transferase Smurf2 Smad Ubiquitination Regulatory Factor 2 Smad-Specific E3 Ubiquitin-Protein Ligase 2 |
| Database Links | Reactome: R-HSA-201451 Reactome: R-HSA-2173788 Reactome: R-HSA-2173795 Reactome: R-HSA-4608870 Reactome: R-HSA-4641257 Reactome: R-HSA-5632684 Reactome: R-HSA-5689880 Reactome: R-HSA-8941858 Reactome: R-HSA-983168 |
| Cellular Localisation | Nucleus Cytoplasm Cell Membrane Membrane Raft Cytoplasmic In The Presence Of Smad7 Colocalizes With Cav1 Smad7 And Tgf-Beta Receptor In Membrane Rafts |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Smurf2 antibody Anti-Hsmurf2 antibody Anti-Hect-Type E3 Ubiquitin Transferase Smurf2 antibody Anti-Smad Ubiquitination Regulatory Factor 2 antibody Anti-Smad-Specific E3 Ubiquitin-Protein Ligase 2 antibody Anti-SMURF2 antibody |
Information sourced from Uniprot.org