| Host: | Rabbit |
| Applications: | WB/ICC/IP |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-nSMase3 (C-Term) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000 ICC 1:250 IP 1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Smpd4 |
| Gene ID: | 77626 |
| Uniprot ID: | NSMA3_MOUSE |
| Immunogen Region: | C-Term |
| Specificity: | The antibody detects an 85-90 kDa* band corresponding to nSMase3 on SDS-PAGE immunoblots of adult mouse heart and mouse C2C12 cells. The antibody works for western blot, ELISA, immunoprecipitation, and immunocytochemistry. |
| Immunogen: | nSMase3 synthetic peptide corresponds to amino acids in the C-terminal region of mouse nSMase3. This sequence is highly conserved in rat and human nSMase3, and has low homology to other nSMase family members. |
| Function | Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. It has a relevant role in the homeostasis of membrane sphingolipids, thereby influencing membrane integrity, and endoplasmic reticulum organization and function. May sensitize cells to DNA damage-induced apoptosis. In skeletal muscle, mediates TNF-stimulated oxidant production. |
| Protein Name | Sphingomyelin Phosphodiesterase 4 Neutral Sphingomyelinase 3 Nsmase-3 Nsmase3 Neutral Sphingomyelinase Iii |
| Database Links | Reactome: R-MMU-9840310 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Golgi Apparatus Membrane Nucleus Envelope Cell Membrane Sarcolemma |
| Alternative Antibody Names | Anti-Sphingomyelin Phosphodiesterase 4 antibody Anti-Neutral Sphingomyelinase 3 antibody Anti-Nsmase-3 antibody Anti-Nsmase3 antibody Anti-Neutral Sphingomyelinase Iii antibody Anti-Smpd4 antibody Anti-Kiaa1418 antibody |
Information sourced from Uniprot.org