| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SMPD1/ASM (70-631) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMPD1 |
| Gene ID: |
6609 |
| Uniprot ID: |
ASM_HUMAN |
| Immunogen Region: |
70-631 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 70-631 of human SMPD1/ASM (NP_000534.3). |
| Immunogen Sequence: |
PARLHRIVPRLRDVFGWGNL TCPICKGLFTAINLGLKKEP NVARVGSVAIKLCNLLKIAP PAVCQSIVHLFEDDMVEVWR RSVLSPSEACGLLLGSTCGH WDIFSSWNISLPTVPKPPPK PPSPPAPGAPVSRILFLTDL HWDHDYLEGTDPDCADPLCC RRGSGLPPASRPGAGYWGEY SKCDLPLRTLESLLSGLGPA GPFDMVYWTGDIPAHDVWHQ TRQDQLRALTTVTALVRKF |
| Post Translational Modifications | Proteolytically processed. Mature lysosomal form arises from C-terminal proteolytic processing of pro-sphingomyelin phosphodiesterase. Sphingomyelin phosphodiesterase, processed form: This form is generated following cleavage by CASP7 in the extracellular milieu. It shows increased activity. Both lysosomal and secreted forms are glycosylated but they show a differential pattern of glycosylation. Phosphorylated at Ser-510 by PRKCD upon stress stimuli. Phosphorylation is required for secretion. |
| Function | Converts sphingomyelin to ceramide. Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly. However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form. In the lysosomes, converts sphingomyelin to ceramide. Plays an important role in the export of cholesterol from the intraendolysosomal membranes. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Modulates stress-induced apoptosis through the production of ceramide. When secreted, modulates cell signaling with its ability to reorganize the plasma membrane by converting sphingomyelin to ceramide. Secreted form is increased in response to stress and inflammatory mediators such as IL1B, IFNG or TNF as well as upon infection with bacteria and viruses. Produces the release of ceramide in the outer leaflet of the plasma membrane playing a central role in host defense. Ceramide reorganizes these rafts into larger signaling platforms that are required to internalize P. aeruginosa, induce apoptosis and regulate the cytokine response in infected cells. In wounded cells, the lysosomal form is released extracellularly in the presence of Ca(2+) and promotes endocytosis and plasma membrane repair. Sphingomyelin phosphodiesterase, processed form: This form is generated following cleavage by CASP7 in the extracellular milieu in response to bacterial infection. It shows increased ability to convert sphingomyelin to ceramide and promotes plasma membrane repair. Plasma membrane repair by ceramide counteracts the action of gasdermin-D (GSDMD) perforin (PRF1) pores that are formed in response to bacterial infection. (Microbial infection) Secretion is activated by bacteria such as P. aeruginos, N. gonorrhoeae and others, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection. (Microbial infection) Secretion is activated by human coronaviruses SARS-CoV and SARS-CoV-2 as well as Zaire ebolavirus, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection. Isoform 2: Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity. Isoform 3: Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity. |
| Protein Name | Sphingomyelin PhosphodiesteraseAcid SphingomyelinaseAsmase Cleaved Into - Sphingomyelin Phosphodiesterase - Processed Form |
| Database Links | Reactome: R-HSA-1660662 |
| Cellular Localisation | LysosomeLipid DropletSecretedThe Secreted Form Is Induced In A Time- And Dose-Dependent By Il1b And Tnf As Well As Stress And Viral InfectionThis Increase Of The Secreted Form Seems To Be Due To Exocytosis Of The Lysosomal Form And Is Ca(2+)-DependentSecretion Is Dependent Of Phosphorylation At Ser-510Secretion Is Induced By Inflammatory Mediators Such As Il1bIfng Or Tnf As Well As Infection With Bacteria And VirusesSphingomyelin PhosphodiesteraseProcessed Form: SecretedExtracellular SpaceThis Form Is Generated Following Cleavage By Casp7 |
| Alternative Antibody Names | Anti-Sphingomyelin Phosphodiesterase antibodyAnti-Acid Sphingomyelinase antibodyAnti-Asmase Cleaved Into - Sphingomyelin Phosphodiesterase - Processed Form antibodyAnti-SMPD1 antibodyAnti-ASM antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
