• Western blot analysis of extracts of various cell lines, using SMEK1 antibody (STJ110798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SMEK1 Rabbit polyclonal antibody (STJ110798) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using SMEK1 rabbit polyclonal antibody (STJ110798) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SMEK1 Rabbit polyclonal antibody (STJ110798) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using SMEK1 rabbit polyclonal antibody (STJ110798) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SMEK1 Rabbit polyclonal antibody (STJ110798) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using SMEK1 rabbit polyclonal antibody (STJ110798) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using SMEK1 antibody (STJ110798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMEK1 antibody (395-594) (STJ110798)

SKU:
STJ110798

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMEK1 (395-594) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP4R3A
Gene ID: 55671
Uniprot ID: P4R3A_HUMAN
Immunogen Region: 395-594
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_001271210.1).
Immunogen Sequence: VQTFKGLKLRFEQQRERQDN PKLDSMRSILRNHRYRRDAR TLEDEEEMWFNTDEDDMEDG EAVVSPSDKTKNDDDIMDPI SKFMERKKLKESEEKEVLLK TNLSGRQSPSFKLSLSSGTK TNLTSQSSTTNLPGSPGSPG SPGSPGSPGSVPKNTSQTAA ITTKGGLVGLVDYPDDDEDD DEDEDKEDTLPLSKKAKFDS
Function Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair.
Protein Name Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 3a
Smek Homolog 1
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
In Interphase Localized In The Cytoplasm And In The Nucleus (With Higher Levels)
During Metaphase Located In Pericentriolar Regions
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 3a antibody
Anti-Smek Homolog 1 antibody
Anti-PPP4R3A antibody
Anti-KIAA2010 antibody
Anti-PP4R3A antibody
Anti-SMEK1 antibody
Anti-MSTP033 antibody

Information sourced from Uniprot.org

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