Anti-SMAD9 antibody (Internal) (STJ70900)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70900-100
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General Information

Short DescriptionGoat polyclonal antibody anti-SMAD9 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
ApplicationsPep-ELISA/WB/IHC
HostGoat
ReactivityHuman/Mouse/Rat/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:64000.
WB: Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 µg/ml. Primary incubation was 1 hour.
IHC:
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolSMAD9
Gene ID4093
Uniprot IDSMAD9_HUMAN
Accession NumberNP_005896.1
Immunogen RegionInternal
Immunogen SequenceHSEPLMPHNATYPD
SpecificityThis antibody is not expected to cross-react with SMAD1 and SMAD5.

Additional Info

Post Translational Modifications Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Protein Name Mothers Against Decapentaplegic Homolog 9
Mad Homolog 9
Mothers Against Dpp Homolog 9
Madh6
Smad Family Member 9
Smad 9
Smad9
Database Links Reactome: R-HSA-201451
Cellular Localisation Cytoplasm
Nucleus
In The Cytoplasm In The Absence Of Ligand
Migration To The Nucleus When Complexed With Smad4
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 9 antibody
Anti-Mad Homolog 9 antibody
Anti-Mothers Against Dpp Homolog 9 antibody
Anti-Madh6 antibody
Anti-Smad Family Member 9 antibody
Anti-Smad 9 antibody
Anti-Smad9 antibody
Anti-SMAD9 antibody
Anti-MADH6 antibody
Anti-MADH9 antibody
Anti-SMAD8 antibody

Information sourced from Uniprot.org

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