• Immunohistochemical analysis of paraffin-embedded human-uterus, antibody was diluted at 1:100
  • Western blot analysis of extracts from K562 cells, using Smad5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SMAD5 antibody (Internal) (STJ96452)

SKU:
STJ96452

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mothers against decapentaplegic homolog 5 (Internal) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD5
Gene ID: 4090
Uniprot ID: SMAD5_HUMAN
Immunogen Region: Internal
Specificity: Smad5 Polyclonal Antibody detects endogenous levels of Smad5 protein.
Immunogen: Synthesized peptide derived from the Internal region of human Smad5.
Post Translational Modifications Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Function Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis.
Protein Name Mothers Against Decapentaplegic Homolog 5
Mad Homolog 5
Mothers Against Dpp Homolog 5
Jv5-1
Smad Family Member 5
Smad 5
Smad5
Hsmad5
Database Links Reactome: R-HSA-201451
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Cytoplasmic In The Absence Of Ligand
Migrates To The Nucleus When Complexed With Smad4
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 5 antibody
Anti-Mad Homolog 5 antibody
Anti-Mothers Against Dpp Homolog 5 antibody
Anti-Jv5-1 antibody
Anti-Smad Family Member 5 antibody
Anti-Smad 5 antibody
Anti-Smad5 antibody
Anti-Hsmad5 antibody
Anti-SMAD5 antibody
Anti-MADH5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance