Anti-SMAD5 antibody (244-262 aa) [R07-2A5] (STJA0011975)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human SMAD5
General Information
| Short Description | Rabbit monoclonal anti-Smad5 (244-262 aa) for use in WB, IHC-F, IHC-P, ICC and IF in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-2A5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4ยฐC short term. Aliquot and store at-20ยฐC long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SMAD5 |
| Gene ID | 4090 |
| Uniprot ID | SMAD5_HUMAN |
| Immunogen | A synthetic peptide of human SMAD5 |
| Immunogen Region | 244-262 aa |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. |
| Function | Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis. |
| Protein Name | Mothers Against Decapentaplegic Homolog 5Mad Homolog 5Mothers Against Dpp Homolog 5Jv5-1Smad Family Member 5Smad 5Smad5Hsmad5 |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | CytoplasmNucleusMitochondrionCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4 |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 5 antibodyAnti-Mad Homolog 5 antibodyAnti-Mothers Against Dpp Homolog 5 antibodyAnti-Jv5-1 antibodyAnti-Smad Family Member 5 antibodyAnti-Smad 5 antibodyAnti-Smad5 antibodyAnti-Hsmad5 antibodyAnti-SMAD5 antibodyAnti-MADH5 antibody |
Information sourced from Uniprot.org