| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMAD5 |
| Gene ID: | 4090 |
| Uniprot ID: | SMAD5_HUMAN |
| Immunogen Region: | 186-465 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 186-465 of human Smad5 (NP_005894.3). |
| Immunogen Sequence: | PLSPNSPYPPSPASSTYPNS PASSGPGSPFQLPADTPPPA YMPPDDQMGQDNSQPMDTSN NMIPQIMPSISSRDVQPVAY EEPKHWCSIVYYELNNRVGE AFHASSTSVLVDGFTDPSNN KSRFCLGLLSNVNRNSTIEN TRRHIGKGVHLYYVGGEVYA ECLSDSSIFVQSRNCNFHHG FHPTTVCKIPSSCSLKIFNN QEFAQLLAQSVNHGFEAVYE LTKMCTIRMSFVKGWGAEY |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. |
| Function | Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis. |
| Protein Name | Mothers Against Decapentaplegic Homolog 5 Mad Homolog 5 Mothers Against Dpp Homolog 5 Jv5-1 Smad Family Member 5 Smad 5 Smad5 Hsmad5 |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | Cytoplasm Nucleus Mitochondrion Cytoplasmic In The Absence Of Ligand Migrates To The Nucleus When Complexed With Smad4 |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 5 antibody Anti-Mad Homolog 5 antibody Anti-Mothers Against Dpp Homolog 5 antibody Anti-Jv5-1 antibody Anti-Smad Family Member 5 antibody Anti-Smad 5 antibody Anti-Smad5 antibody Anti-Hsmad5 antibody Anti-SMAD5 antibody Anti-MADH5 antibody |
Information sourced from Uniprot.org