| Host: | Rabbit |
| Applications: | WB/IHC-P/IP/ELISA/ChIP/CUT&Tag |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Monoclonal |
| Clone ID: | S3MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 IHC-P:1:500-1:2000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMAD4 |
| Gene ID: | 4089 |
| Uniprot ID: | SMAD4_HUMAN |
| Immunogen Region: | 320-552 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1). |
| Immunogen Sequence: | PEYWCSIAYFEMDVQVGETF KVPSSCPIVTVDGYVDPSGG DRFCLGQLSNVHRTEAIERA RLHIGKGVQLECKGEGDVWV RCLSDHAVFVQSYYLDREAG RAPGDAVHKIYPSAYIKVFD LRQCHRQMQQQAATAQAAAA AQAAAVAGNIPGPGSVGGIA PAISLSAAAGIGVDDLRRLC ILRMSFVKGWGPDYPRQSIK ETPCWIEIHLHRALQLLDEV LHTMPIADPQPLD |
| Post Translational Modifications | Phosphorylated by PDPK1. Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling. |
| Function | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site.required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 4 Mad Homolog 4 Mothers Against Dpp Homolog 4 Deletion Target In Pancreatic Carcinoma 4 Smad Family Member 4 Smad 4 Smad4 Hsmad4 |
| Database Links | Reactome: R-HSA-1181150 Reactome: R-HSA-1502540 Reactome: R-HSA-201451 Reactome: R-HSA-2173789 Reactome: R-HSA-2173795 Reactome: R-HSA-2173796 Reactome: R-HSA-3311021 Reactome: R-HSA-3315487 Reactome: R-HSA-452723 Reactome: R-HSA-5689880 Reactome: R-HSA-8941326 Reactome: R-HSA-8941855 Reactome: R-HSA-8952158 Reactome: R-HSA-9615017 Reactome: R-HSA-9617828 Reactome: R-HSA-9733709 Reactome: R-HSA-9735871 Reactome: R-HSA-9754189 Reactome: R-HSA-9823730 Reactome: R-HSA-9839394 Reactome: R-HSA-9844594 |
| Cellular Localisation | Cytoplasm Nucleus Cytoplasmic In The Absence Of Ligand Migrates To The Nucleus When Complexed With R-Smad Pdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 4 antibody Anti-Mad Homolog 4 antibody Anti-Mothers Against Dpp Homolog 4 antibody Anti-Deletion Target In Pancreatic Carcinoma 4 antibody Anti-Smad Family Member 4 antibody Anti-Smad 4 antibody Anti-Smad4 antibody Anti-Hsmad4 antibody Anti-SMAD4 antibody Anti-DPC4 antibody Anti-MADH4 antibody |
Information sourced from Uniprot.org
