Anti-Smad1/5/9 antibody (10-59 aa) (STJ95690)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Smad1/5/9 at the amino acid range 10-59
General Information
| Short Description | Rabbit polyclonal anti-Mothers against decapentaplegic homolog 1 and Mothers against decapentaplegic homolog 5 and Mothers against decapentaplegic homolog 9 (10-59 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet includ |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMAD9SMAD1409340864090SMAD9_HUMANSMAD1_HUMANSMAD5_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Smad1/5/9 at the amino acid range 10-59 |
| Immunogen Region | 10-59 aa |
| Specificity | Smad1/5/9 Polyclonal Antibody detects endogenous levels of Smad1/5/9 protein. |
Additional Info
| Post Translational Modifications | Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. |
| Protein Name | Mothers Against Decapentaplegic Homolog 1Mad Homolog 1Mothers Against Dpp Homolog 1Jv4-1Mad-Related Protein 1Smad Family Member 1Smad 1Smad1Hsmad1Transforming Growth Factor-Beta-Signaling Protein 1Bsp-1 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-5689880Reactome: R-HSA-8941326 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4Co-Localizes With Lemd3 At The Nucleus Inner MembraneExported From The Nucleus To The Cytoplasm When Dephosphorylated |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 1 antibodyAnti-Mad Homolog 1 antibodyAnti-Mothers Against Dpp Homolog 1 antibodyAnti-Jv4-1 antibodyAnti-Mad-Related Protein 1 antibodyAnti-Smad Family Member 1 antibodyAnti-Smad 1 antibodyAnti-Smad1 antibodyAnti-Hsmad1 antibodyAnti-Transforming Growth Factor-Beta-Signaling Protein 1 antibodyAnti-Bsp-1 antibodyAnti-SMAD1 antibodyAnti-BSP1 antibodyAnti-MADH1 antibodyAnti-MADR1 antibody |
Information sourced from Uniprot.org