| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Mothers against decapentaplegic homolog 1 and Mothers against decapentaplegic homolog 5 and Mothers against decapentaplegic homolog 9 (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluoresc |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMAD5SMAD9409040934086SMAD5_HUMANSMAD9_HUMANSMAD1_HUMAN |
| Immunogen Region: |
10-59 aa |
| Specificity: |
Smad1/5/9 Polyclonal Antibody detects endogenous levels of Smad1/5/9 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Smad1/5/9 at the amino acid range 10-59 |
| Post Translational Modifications | Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. |
| Protein Name | Mothers Against Decapentaplegic Homolog 1Mad Homolog 1Mothers Against Dpp Homolog 1Jv4-1Mad-Related Protein 1Smad Family Member 1Smad 1Smad1Hsmad1Transforming Growth Factor-Beta-Signaling Protein 1Bsp-1 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-5689880Reactome: R-HSA-8941326 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4Co-Localizes With Lemd3 At The Nucleus Inner MembraneExported From The Nucleus To The Cytoplasm When Dephosphorylated |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 1 antibodyAnti-Mad Homolog 1 antibodyAnti-Mothers Against Dpp Homolog 1 antibodyAnti-Jv4-1 antibodyAnti-Mad-Related Protein 1 antibodyAnti-Smad Family Member 1 antibodyAnti-Smad 1 antibodyAnti-Smad1 antibodyAnti-Hsmad1 antibodyAnti-Transforming Growth Factor-Beta-Signaling Protein 1 antibodyAnti-Bsp-1 antibodyAnti-SMAD1 antibodyAnti-BSP1 antibodyAnti-MADH1 antibodyAnti-MADR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
