| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC7A5 (1-125) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC7A5 |
| Gene ID: |
8140 |
| Uniprot ID: |
LAT1_HUMAN |
| Immunogen Region: |
1-125 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LAT1/SLC7A5 (NP_003477.4). |
| Immunogen Sequence: |
MAGAGPKRRALAAPAAEEKE EAREKMLAAKSADGSAPAGE GEGVTLQRNITLLNGVAIIV GTIIGSGIFVTPTGVLKEAG SPGLALVVWAACGVFSIVGA LCYAELGTTISKSGGDYAYM LEVYG |
| Tissue Specificity | Detected in placenta, in the syncytiotrophoblast layer (at protein level). Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. |
| Function | The heterodimer with SLC3A2 functions as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, leucine, histidine, methionine, tryptophan, valine, isoleucine and alanine. The heterodimer with SLC3A2 mediates the uptake of L-DOPA. Functions as an amino acid exchanger. May play a role in the transport of L-DOPA across the blood-brain barrier. May act as the major transporter of tyrosine in fibroblasts (Probable). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier.Can mediate the transport of thyroid hormones diiodothyronine (T2), triiodothyronine (T3) and thyroxine (T4) across the cell membrane. When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the membrane. (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis. |
| Protein Name | Large Neutral Amino Acids Transporter Small Subunit 14f2 Light Chain4f2 Lc4f2lcCd98 Light ChainIntegral Membrane Protein E16E16L-Type Amino Acid Transporter 1Hlat1Solute Carrier Family 7 Member 5Y+ System Cationic Amino Acid Transporter |
| Database Links | Reactome: R-HSA-210991Reactome: R-HSA-352230Reactome: R-HSA-71240 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell MembraneLysosome MembraneLocated To The Plasma Membrane By Slc3a2/4f2hcLocalized To The Apical Membrane Of Placental Syncytiotrophoblastic CellsRecruited To Lysosomes By Laptm4b |
| Alternative Antibody Names | Anti-Large Neutral Amino Acids Transporter Small Subunit 1 antibodyAnti-4f2 Light Chain antibodyAnti-4f2 Lc antibodyAnti-4f2lc antibodyAnti-Cd98 Light Chain antibodyAnti-Integral Membrane Protein E16 antibodyAnti-E16 antibodyAnti-L-Type Amino Acid Transporter 1 antibodyAnti-Hlat1 antibodyAnti-Solute Carrier Family 7 Member 5 antibodyAnti-Y+ System Cationic Amino Acid Transporter antibodyAnti-SLC7A5 antibodyAnti-CD98LC antibodyAnti-LAT1 antibodyAnti-MPE16 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
