• Western blot analysis of 293F, using SLC5A1 Rabbit polyclonal antibody (STJ113885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-SLC5A1 antibody (408-422) (STJ113885)

SKU:
STJ113885

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC5A1 (408-422) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC5A1
Gene ID: 6523
Uniprot ID: SC5A1_HUMAN
Immunogen Region: 408-422
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 408-422 of human SLC5A1 (NP_000334.1).
Immunogen Sequence: DIYAKVRKRASEKEL
Tissue Specificity Expressed in intestine. Expressed in endometrial cells.
Post Translational Modifications N-glycosylation is not necessary for the cotransporter function.
Function Electrogenic Na(+)-coupled sugar simporter that actively transports D-glucose or D-galactose at the plasma membrane, with a Na(+) to sugar coupling ratio of 2:1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump. Has a primary role in the transport of dietary monosaccharides from enterocytes to blood. Responsible for the absorption of D-glucose or D-galactose across the apical brush-border membrane of enterocytes, whereas basolateral exit is provided by GLUT2. Additionally, functions as a D-glucose sensor in enteroendocrine cells, triggering the secretion of the incretins GCG and GIP that control food intake and energy homeostasis. Together with SGLT2, functions in reabsorption of D-glucose from glomerular filtrate, playing a nonredundant role in the S3 segment of the proximal tubules. Transports D-glucose into endometrial epithelial cells, controlling glycogen synthesis and nutritional support for the embryo as well as the decidual transformation of endometrium prior to conception. Acts as a water channel enabling passive water transport across the plasma membrane in response to the osmotic gradient created upon sugar and Na(+) uptake. Has high water conductivity, comparable to aquaporins, and therefore is expected to play an important role in transepithelial water permeability, especially in the small intestine.
Protein Name Sodium/Glucose Cotransporter 1
Na(+/Glucose Cotransporter 1
High Affinity Sodium-Glucose Cotransporter
Solute Carrier Family 5 Member 1
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-5656364
Reactome: R-HSA-8981373
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Sodium/Glucose Cotransporter 1 antibody
Anti-Na(+/Glucose Cotransporter 1 antibody
Anti-High Affinity Sodium-Glucose Cotransporter antibody
Anti-Solute Carrier Family 5 Member 1 antibody
Anti-SLC5A1 antibody
Anti-NAGT antibody
Anti-SGLT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance