| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC46A1 (350-450 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
SLC46A1 |
| Gene ID: |
113235 |
| Uniprot ID: |
PCFT_HUMAN |
| Immunogen Region: |
350-450 aa |
| Specificity: |
Specific for SLC46A1. |
| Immunogen: |
A synthetic peptide from aa region 350-450 of human SLC46A1 conjugated to an immunogenic carrier protein was used as the antigen. |
| Function | Proton-coupled folate symporter that mediates folate absorption using an H(+) gradient as a driving force. Involved in the intestinal absorption of folates at the brush-border membrane of the proximal jejunum, and the transport from blood to cerebrospinal fluid across the choroid plexus. Functions at acidic pH via alternate outward- and inward-open conformation states. Protonation of residues in the outward open state primes the protein for transport. Binding of folate promotes breaking of salt bridge network and subsequent closure of the extracellular gate, leading to the inward-open state and release of protons and folate. Also able to transport antifolate drugs, such as methotrexate and pemetrexed, which are established treatments for cancer and autoimmune diseases. Involved in FOLR1-mediated endocytosis by serving as a route of export of folates from acidified endosomes. Also acts as a lower-affinity, pH-independent heme carrier protein and constitutes the main importer of heme in the intestine. Imports heme in the retina and retinal pigment epithelium, in neurons of the hippocampus, in hepatocytes and in the renal epithelial cells. Hence, participates in the trafficking of heme and increases intracellular iron content. Isoform 2: Inactive isoform which is not able to mediate proton-coupled folate transport. |
| Protein Name | Proton-Coupled Folate TransporterHspcftHpcftHeme Carrier Protein 1Pcft/Hcp1Solute Carrier Family 46 Member 1 |
| Database Links | Reactome: R-HSA-196757Reactome: R-HSA-917937Reactome: R-HSA-9707616 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneBasolateral Cell MembraneEndosome MembraneCytoplasmLocalizes To The Apical Membrane Of Intestinal Cells In Iron-Deficient CellsWhile It Resides In The Cytoplasm In Iron-Replete CellsLocalizes To The Basolateral Membrane Of Choroid Plexus |
| Alternative Antibody Names | Anti-Proton-Coupled Folate Transporter antibodyAnti-Hspcft antibodyAnti-Hpcft antibodyAnti-Heme Carrier Protein 1 antibodyAnti-Pcft/Hcp1 antibodyAnti-Solute Carrier Family 46 Member 1 antibodyAnti-SLC46A1 antibodyAnti-G21 antibodyAnti-HCP1 antibodyAnti-PCFT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
