| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC3A2 (Internal Region) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
SLC3A2 |
| Gene ID: |
6520 |
| Uniprot ID: |
4F2_HUMAN |
| Immunogen Region: |
Internal Region |
| Specificity: |
Specific for SLC3A2. |
| Immunogen: |
A synthetic peptide from internal region of human CD98 (SLC3A2, 4F2 heavy chain antigen, MDU1) conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | N-glycosylated.N-glycosylation is crucial for trafficking and stability of SLC3A2 to the plasma membrane. Phosphorylation on Ser-406.Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions. |
| Function | Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters. Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids. Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane. SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA. SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression. (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis. (Microbial infection) Acts as a receptor for malaria parasite Plasmodium vivax (Thai isolate) in immature red blood cells. |
| Protein Name | Amino Acid Transporter Heavy Chain Slc3a24f2 Cell-Surface Antigen Heavy Chain4f2hc4f2 Heavy Chain AntigenLymphocyte Activation Antigen 4f2 Large SubunitSolute Carrier Family 3 Member 2Cd Antigen Cd98 |
| Database Links | Reactome: R-HSA-210991Reactome: R-HSA-352230Reactome: R-HSA-5660862Reactome: R-HSA-71240 |
| Cellular Localisation | Apical Cell MembraneCell MembraneSingle-Pass Type Ii Membrane ProteinCell JunctionLysosome MembraneMelanosomeBasolateral Cell MembraneLocalized At The Plasma Membrane When Associated With Slc7a5/Lat1 Or Slc7a8/Lat2Localized To The Apical Membrane Of Placental Syncytiotrophoblastic CellsRecruited To Lysosomes By Laptm4bIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocated Selectively At Cell-Cell Adhesion SitesColocalized With Slc7a8/Lat2 At The Basolateral Membrane Of Kidney Proximal Tubules And Small Intestine EpitheliaExpressed In Both Luminal And Abluminal Membranes Of Brain Capillary Endothelial Cells |
| Alternative Antibody Names | Anti-Amino Acid Transporter Heavy Chain Slc3a2 antibodyAnti-4f2 Cell-Surface Antigen Heavy Chain antibodyAnti-4f2hc antibodyAnti-4f2 Heavy Chain Antigen antibodyAnti-Lymphocyte Activation Antigen 4f2 Large Subunit antibodyAnti-Solute Carrier Family 3 Member 2 antibodyAnti-Cd Antigen Cd98 antibodyAnti-SLC3A2 antibodyAnti-MDU1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
