Host: | Rabbit |
Applications: | ELISA/IHC/IP/WB |
Reactivity: | Human/Monkey/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-SLC31A1 (1-100) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | FITC |
Isotype: | IgG |
Purification: | Affinity Purified |
Concentration: | 0.68 µg/µl |
Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:50 |
Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Gene Symbol: | SLC31A1 |
Gene ID: | 1317 |
Uniprot ID: | COPT1_HUMAN |
Immunogen Region: | 1-100 |
Immunogen: | N-epitope synthetic peptide taken within amino acid region 1-100 on human High affinity copper uptake protein 1. |
Post Translational Modifications | O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain. Proteolytic cleavage, leading to a truncated form, is facilitated by SLC31A2 and initiated preferentially by CTSL and to a minor extend by CTSB in endolysosomal compartments. In vitro, is cleaved by CTSL/cathepsin L between residues 8 and 9 from the amino terminus. A post-CTSL/cathepsin L processing occurs to yield to the fully truncated form. Sulfenylated at Cys-189 after stimulation with VEGFA, which induces SLC31A1-KDR disulfide bond formation and their co-internalization to early endosomes, driving to a sustained VEGFR2 signaling. |
Function | High affinity copper uptake protein 1: Uniporter that mediates the transport of copper(1+) from the extracellular space to the cytoplasm, across the plasma membrane and delivers directly copper(1+) to specific chaperone such as ATOX1, via a copper(1+)- mediated transient interaction between the C-terminal domain and a copper(1+) chaperone, thus controlling intracellular copper(1+) levels. May function in copper(1+) import from the apical membrane thus may drive intestinal copper absorption. The copper(1+) transport mechanism is sodium-independent, saturable and of high-affinity. Also mediates the uptake of silver(1+). May function in the influx of the platinum-containing chemotherapeutic agents. The platinum-containing chemotherapeutic agents uptake is saturable. In vitro, mediates the transport of cadmium(2+) into cells. Also participates in the first step of copper(2+) acquisition by cells through a direct transfer of copper(2+) from copper(2+) carriers in blood, such as ALB to the N-terminal domain of SLC31A1, leading to copper(2+) reduction and probably followed by copper(1+) stabilization. In addition, functions as a redox sensor to promote angiogenesis in endothelial cells, in a copper(1+) transport independent manner, by transmitting the VEGF-induced ROS signal through a sulfenylation at Cys-189 leadin g to a subsequent disulfide bond formation between SLC31A1 and KDR. The SLC31A1-KDR complex is then co-internalized to early endosomes, driving a sustained VEGFR2 signaling. Truncated CTR1 form: Mobilizes copper(1+) out of the endosomal compartment, making copper(1+) available for export out of the cells. |
Protein Name | High Affinity Copper Uptake Protein 1 Copper Transporter 1 Hctr1 Solute Carrier Family 31 Member 1 Cleaved Into - Truncated Ctr1 Form |
Database Links | Reactome: R-HSA-425410 |
Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Early Endosome Membrane Recycling Endosome Membrane Apical Cell Membrane Late Endosome Membrane Basolateral Cell Membrane The Localization Is Controlled By The Intra And Extra-Cellular Copper Concentration Under Conditions Of Elevated Extracellular Copper Concentrations It Is Rapidly Internalized By Endocytosis From The Plasma Membrane By A Clathrin- And Dynamin-Mediated Process And Degradated In Order To Prevent Intracellular Copper Accumulation And To Reduce The Transport Of The Copper Across The Membrane The Internalized Slc31a1 Is Then Localized In Early Endosomes And Upon A Low Extracellular Copper Concentrations It Is Transported Back To The Plasma Membrane In A Rab11a-Dependent Recycling Pathway Localizes To The Apical Membrane In Intestinal Epithelial Cells Mainly Localized On The Basolateral Side Of Renal Tubular Cells Localizes To The Neuronal Cell Body Plasma Membranes |
Alternative Antibody Names | Anti-High Affinity Copper Uptake Protein 1 antibody Anti-Copper Transporter 1 antibody Anti-Hctr1 antibody Anti-Solute Carrier Family 31 Member 1 Cleaved Into - Truncated Ctr1 Form antibody Anti-SLC31A1 antibody Anti-COPT1 antibody Anti-CTR1 antibody |
Information sourced from Uniprot.org