• Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of SKOV3 cells using Glut4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-SLC2A4 antibody (21-70 aa) (STJ96888)

SKU:
STJ96888

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Solute carrier family 2, facilitated glucose transporter member 4 (21-70 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:100-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC2A4
Gene ID: 6517
Uniprot ID: GLUT4_HUMAN
Immunogen Region: 21-70 aa
Specificity: Glut4 Polyclonal Antibody detects endogenous levels of Glut4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the N-terminal region of human SLC2A4 at the amino acid range 21-70
Post Translational Modifications Sumoylated. Palmitoylated. Palmitoylation by ZDHHC7 controls the insulin-dependent translocation of GLUT4 to the plasma membrane.
Function Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.
Protein Name Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4
Glucose Transporter Type 4 - Insulin-Responsive
Glut-4
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-189200
Reactome: R-HSA-381340
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endomembrane System
Cytoplasm
Perinuclear Region
Localizes Primarily To The Perinuclear Region
Undergoing Continued Recycling To The Plasma Membrane Where It Is Rapidly Reinternalized
The Dileucine Internalization Motif Is Critical For Intracellular Sequestration
Insulin Stimulation Induces Translocation To The Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 antibody
Anti-Glucose Transporter Type 4 - Insulin-Responsive antibody
Anti-Glut-4 antibody
Anti-SLC2A4 antibody
Anti-GLUT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance