• Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and SLC25A22 knockout (KO) 293T cells, using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SLC25A22 antibody (128-188) (STJ119808)

SKU:
STJ119808

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC25A22
Gene ID: 79751
Uniprot ID: GHC1_HUMAN
Immunogen Region: 128-188
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 128-188 of human SLC25A22 (NP_078974.1).
Immunogen Sequence: KIQLQDAGRIAAQRKILAAQ GQLSAQGGAQPSVEAPAAPR PTATQLTRDLLRSRGIAGLY K
Tissue Specificity Expressed at high levels in brain, liver, and pancreas.
Function Mitochondrial glutamate/H(+) symporter. Responsible for the transport of glutamate from the cytosol into the mitochondrial matrix with the concomitant import of a proton. Plays a role in the control of glucose-stimulated insulin secretion.
Protein Name Mitochondrial Glutamate Carrier 1
Gc-1
Glutamate/H(+ Symporter 1
Solute Carrier Family 25 Member 22
Database Links Reactome: R-HSA-428643
Reactome: R-HSA-9856872
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Glutamate Carrier 1 antibody
Anti-Gc-1 antibody
Anti-Glutamate/H(+ Symporter 1 antibody
Anti-Solute Carrier Family 25 Member 22 antibody
Anti-SLC25A22 antibody
Anti-GC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance