![Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98597/382525/STJ119808_1__29834.1713150947.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98597/382526/STJ119808_2__89377.1713150947.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and SLC25A22 knockout (KO) 293T cells, using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98597/382527/STJ119808_3__39869.1713150949.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SLC25A22 knockout (KO) 293T cells, using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of various lysates using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98597/382528/STJ119808_4__34140.1713150950.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98597/382525/STJ119808_1__29834.1713150947.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SLC25A22 |
| Gene ID: | 79751 |
| Uniprot ID: | GHC1_HUMAN |
| Immunogen Region: | 128-188 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 128-188 of human SLC25A22 (NP_078974.1). |
| Immunogen Sequence: | KIQLQDAGRIAAQRKILAAQ GQLSAQGGAQPSVEAPAAPR PTATQLTRDLLRSRGIAGLY K |
| Tissue Specificity | Expressed at high levels in brain, liver, and pancreas. |
| Function | Mitochondrial glutamate/H(+) symporter. Responsible for the transport of glutamate from the cytosol into the mitochondrial matrix with the concomitant import of a proton. Plays a role in the control of glucose-stimulated insulin secretion. |
| Protein Name | Mitochondrial Glutamate Carrier 1Gc-1Glutamate/H(+ Symporter 1Solute Carrier Family 25 Member 22 |
| Database Links | Reactome: R-HSA-428643Reactome: R-HSA-9856872 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Mitochondrial Glutamate Carrier 1 antibodyAnti-Gc-1 antibodyAnti-Glutamate/H(+ Symporter 1 antibodyAnti-Solute Carrier Family 25 Member 22 antibodyAnti-SLC25A22 antibodyAnti-GC1 antibody |
Information sourced from Uniprot.org