| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC12A5 (20-70 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Slc12a5 |
| Gene ID: |
57138 |
| Uniprot ID: |
S12A5_MOUSE |
| Immunogen Region: |
20-70 aa |
| Specificity: |
Specific for SLC12A5. |
| Immunogen: |
A synthetic peptide from aa region 20-70 of mouse SLC12A5 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and shares 95% identity with human sequence. |
| Post Translational Modifications | Phosphorylated at Thr-929 and Thr-1029 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. |
| Function | Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation. |
| Protein Name | Solute Carrier Family 12 Member 5Electroneutral Potassium-Chloride Cotransporter 2K-Cl Cotransporter 2Mkcc2Neuronal K-Cl Cotransporter |
| Database Links | Reactome: R-MMU-426117 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionDendriteDetected On DendritesBut Not On Axons Of Spinal Cord Neurons And At Gphn-Positive Inhibitory Synapses |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 5 antibodyAnti-Electroneutral Potassium-Chloride Cotransporter 2 antibodyAnti-K-Cl Cotransporter 2 antibodyAnti-Mkcc2 antibodyAnti-Neuronal K-Cl Cotransporter antibodyAnti-Slc12a5 antibodyAnti-Kcc2 antibodyAnti-Kiaa1176 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
