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Anti-SIN3A antibody (1-120) (STJ25530)

Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g Sin3A antibody (STJ25530). Western blot was performed from the immunoprecipitate using Sin3A antibody (STJ25530) at a dilution of 1:1000.
Immunofluorescence analysis of U2OS cells using Sin3A Rabbit polyclonal antibody (STJ25530). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using Sin3A Rabbit polyclonal antibody (STJ25530) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SIN3A antibody (1-120) (STJ25530)

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STJ25530

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IF/IP
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-SIN3A (1-120) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:100-1:500 IF/ICC 1:50-1:200 IP 1:500-1:1000 ChIP 1:20-1:100
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	SIN3A
Gene ID:	25942
Uniprot ID:	SIN3A_HUMAN
Immunogen Region:	1-120
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-120 of human Sin3A (NP_056292.1).
Immunogen Sequence:	MKRRLDDQESPVYAAQQRRI PGSTEAFPHQHRVLAPAPPV YEAVSETMQSATGIQYSVTP SYQVSAMPQSSGSHGPAIAA VHSSHHHPTAVQPHGGQVVQ SHAHPAPPVAPVQGQQQFQR

Tissue Specificity	Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.
Post Translational Modifications	SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
Function	Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.
Protein Name	Paired Amphipathic Helix Protein Sin3a Histone Deacetylase Complex Subunit Sin3a Transcriptional Corepressor Sin3a
Database Links	Reactome: R-HSA-3899300 Reactome: R-HSA-400206 Reactome: R-HSA-427413 Reactome: R-HSA-8936459 Reactome: R-HSA-9022538 Reactome: R-HSA-9022692 Reactome: R-HSA-9022699 Reactome: R-HSA-9022702 Reactome: R-HSA-9615017 Reactome: R-HSA-9701898 Reactome: R-HSA-9707564 Reactome: R-HSA-983231
Cellular Localisation	Nucleus Nucleolus Recruited To The Nucleolus By Sap30l
Alternative Antibody Names	Anti-Paired Amphipathic Helix Protein Sin3a antibody Anti-Histone Deacetylase Complex Subunit Sin3a antibody Anti-Transcriptional Corepressor Sin3a antibody Anti-SIN3A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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