| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-SIGLEC10 (650-697) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.56-0.60 µg/µl |
| Dilution Range: | WB: 1:500 ELISA: 1:10, 000 IP: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | SIGLEC10 |
| Gene ID: | 89790 |
| Uniprot ID: | SIG10_HUMAN |
| Immunogen Region: | 650-697 |
| Immunogen: | Synthetic peptide taken within amino acid region 650-697 on human SIGLEC10 protein. |
| Tissue Specificity | Expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation). Isoform 5 is found to be the most abundant isoform. Found in lymph node, lung, ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord. Isoform 2 is also found in brain. Isoform 4 is specifically found in natural killer cells. |
| Post Translational Modifications | Phosphorylation of Tyr-667 is involved in binding to PTPN6. |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, seems to act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Involved in negative regulation of B-cell antigen receptor signaling. The inhibition of B cell activation is dependent on PTPN6/SHP-1. In association with CD24 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. In association with CD24 may regulate the immune repsonse of natural killer (NK) cells. Plays a role in the control of autoimmunity. During initiation of adaptive immune responses by CD8-alpha(+) dendritic cells inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes. The function seems to implicate recruitment of PTPN6/SHP-1, which dephosphorylates NCF1 of the NADPH oxidase complex consequently promoting phagosomal acidification. |
| Protein Name | Sialic Acid-Binding Ig-Like Lectin 10 Siglec-10 Siglec-Like Protein 2 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Cell Membrane Isoform 3: Cell Membrane Isoform 4: Cell Membrane Isoform 5: Secreted |
| Alternative Antibody Names | Anti-Sialic Acid-Binding Ig-Like Lectin 10 antibody Anti-Siglec-10 antibody Anti-Siglec-Like Protein 2 antibody Anti-SIGLEC10 antibody Anti-SLG2 antibody Anti-UNQ477 antibody Anti-PRO940 antibody |
Information sourced from Uniprot.org