| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | SI |
| Gene ID: | 6476 |
| Uniprot ID: | SUIS_HUMAN |
| Immunogen Region: | 1528-1827 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1528-1827 of human SI (NP_001032.2). |
| Immunogen Sequence: | ADICGFFNNSEYHLCTRWMQ LGAFYPYSRNHNIANTRRQD PASWNETFAEMSRNILNIRY TLLPYFYTQMHEIHANGGTV IRPLLHEFFDEKPTWDIFKQ FLWGPAFMVTPVLEPYVQTV NAYVPNARWFDYHTGKDIGV RGQFQTFNASYDTINLHVRG GHILPCQEPAQNTFYSRQKH MKLIVAADDNQMAQGSLFWD DGESIDTYERDLYLSVQFNL NQTTLTSTILKRGYINKSE |
| Tissue Specificity | Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. |
| Post Translational Modifications | The precursor is proteolytically cleaved when exposed to pancreatic proteases in the intestinal lumen. Sulfated. |
| Function | Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides. |
| Protein Name | Sucrase-Isomaltase - Intestinal Cleaved Into - Sucrase - Isomaltase |
| Database Links | Reactome: R-HSA-189085Reactome: R-HSA-5659898 |
| Cellular Localisation | Apical Cell MembraneSingle-Pass Type Ii Membrane ProteinBrush Border |
| Alternative Antibody Names | Anti-Sucrase-Isomaltase - Intestinal Cleaved Into - Sucrase - Isomaltase antibodyAnti-SI antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89797/358266/STJ11100863_1__40910.1713122607.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")