• Western blot analysis of various lysates, using SHB Rabbit monoclonal antibody (STJ11105276) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilutionLysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SHB antibody (409-509) [S5276RM] (STJ11105276)

SKU:
STJ11105276

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Sh2 Domain-Containing Adapter Protein B (409-509) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5276RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHB
Gene ID: 6461
Uniprot ID: SHB_HUMAN
Immunogen Region: 409-509
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 409-509 of human SHB (NP_003019.2).
Immunogen Sequence: IWYHGAISRGDAENLLRLCK ECSYLVRNSQTSKHDYSLSL RSNQGFMHMKLAKTKEKYVL GQNSPPFDSVPEVIHYYTTR KLPIKGAEHLSLLYPVAVRT L
Tissue Specificity Widely expressed.
Post Translational Modifications Phosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGFR1 or VEGFR2 activation.
Function Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
Protein Name Sh2 Domain-Containing Adapter Protein B
Database Links Reactome: R-HSA-4420097
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Associates With Membrane Lipid Rafts Upon Tcr Stimulation
Alternative Antibody Names Anti-Sh2 Domain-Containing Adapter Protein B antibody
Anti-SHB antibody

Information sourced from Uniprot.org

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