• Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SH3PXD2A antibody (N-Term) (STJ93078)

SKU:
STJ93078

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SH3 and PX domain-containing protein 2A (N-Term) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:100-500
IF ICC 1:100-500
WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SH3PXD2A
Gene ID: 9644
Uniprot ID: SPD2A_HUMAN
Immunogen Region: N-Term
Specificity: Fish Polyclonal Antibody detects endogenous levels of Fish protein.
Immunogen: Synthesized peptide derived from the N-terminal region of human Fish.
Post Translational Modifications Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR.
Function Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.
Protein Name Sh3 And Px Domain-Containing Protein 2a
Adapter Protein Tks5
Five Sh3 Domain-Containing Protein
Sh3 Multiple Domains Protein 1
Tyrosine Kinase Substrate With Five Sh3 Domains
Database Links Reactome: R-HSA-8941237
Reactome: R-HSA-9013148
Cellular Localisation Cytoplasm
Cell Projection
Podosome
Cytoplasmic In Normal Cells And Localizes To Podosomes In Src-Transformed Cells
Alternative Antibody Names Anti-Sh3 And Px Domain-Containing Protein 2a antibody
Anti-Adapter Protein Tks5 antibody
Anti-Five Sh3 Domain-Containing Protein antibody
Anti-Sh3 Multiple Domains Protein 1 antibody
Anti-Tyrosine Kinase Substrate With Five Sh3 Domains antibody
Anti-SH3PXD2A antibody
Anti-FISH antibody
Anti-KIAA0418 antibody
Anti-SH3MD1 antibody
Anti-TKS5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance