• Western blot analysis of extracts from wild type (WT) and 14-3-3 sigma knockout (KO) HeLa (KO) cells, using 14-3-3 sigma antibody (STJ11104343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SFN antibody (1-248) (STJ11104343)

SKU:
STJ11104343

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-14-3-3 Protein Sigma (1-248) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SFN
Gene ID: 2810
Uniprot ID: 1433S_HUMAN
Immunogen Region: 1-248
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human 14-3-3 sigma (NP_006133.1).
Immunogen Sequence: MERASLIQKAKLAEQAERYE DMAAFMKGAVEKGEELSCEE RNLLSVAYKNVVGGQRAAWR VLSSIEQKSNEEGSEEKGPE VREYREKVETELQGVCDTVL GLLDSHLIKEAGDAESRVFY LKMKGDYYRYLAEVATGDDK KRIIDSARSAYQEAMDISKK EMPPTNPIRLGLALNFSVFH YEIANSPEEAISLAKTTFDE AMADLHTLSEDSYKDSTLIM QLLRDNLTLWTADNAGEEG
Tissue Specificity Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Post Translational Modifications Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
Protein Name 14-3-3 Protein Sigma
Epithelial Cell Marker Protein 1
Stratifin
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-6804114
Reactome: R-HSA-75035
Reactome: R-HSA-9614399
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Cytoplasm
Nucleus
Secreted
May Be Secreted By A Non-Classical Secretory Pathway
Alternative Antibody Names Anti-14-3-3 Protein Sigma antibody
Anti-Epithelial Cell Marker Protein 1 antibody
Anti-Stratifin antibody
Anti-SFN antibody
Anti-HME1 antibody

Information sourced from Uniprot.org

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