• Western blot analysis of various lysates using SF3B4 Rabbit polyclonal antibody (STJ119672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SF3B4 antibody (1-200) (STJ119672)

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STJ119672

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SF3B4 (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B4
Gene ID: 10262
Uniprot ID: SF3B4_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SF3B4 (NP_005841.1).
Immunogen Sequence: MAAGPISERNQDATVYVGGL DEKVSEPLLWELFLQAGPVV NTHMPKDRVTGQHQGYGFVE FLSEEDADYAIKIMNMIKLY GKPIRVNKASAHNKNLDVGA NIFIGNLDPEIDEKLLYDTF SAFGVILQTPKIMRDPDTGN SKGYAFINFASFDASDAAIE AMNGQYLCNRPITVSYAFKK DSKGERHGSAAERLLAAQNP
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.
Protein Name Splicing Factor 3b Subunit 4
Pre-Mrna-Splicing Factor Sf3b 49 Kda Subunit
Spliceosome-Associated Protein 49
Sap 49
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 4 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 49 Kda Subunit antibody
Anti-Spliceosome-Associated Protein 49 antibody
Anti-Sap 49 antibody
Anti-SF3B4 antibody
Anti-SAP49 antibody

Information sourced from Uniprot.org

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