| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Splicing factor 3B subunit 1 (75-124 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SF3B1 |
| Gene ID: |
23451 |
| Uniprot ID: |
SF3B1_HUMAN |
| Immunogen Region: |
75-124 aa |
| Specificity: |
SAP 155 Polyclonal Antibody detects endogenous levels of SAP 155 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human SAP 155 at the amino acid range 75-124 |
| Function | Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92. |
| Protein Name | Splicing Factor 3b Subunit 1Pre-Mrna-Splicing Factor Sf3b 155 Kda SubunitSf3b155Spliceosome-Associated Protein 155Sap 155 |
| Database Links | Reactome: R-HSA-5250924Reactome: R-HSA-72163Reactome: R-HSA-72165 |
| Cellular Localisation | NucleusNucleus SpeckleDuring MitosisTransiently Dispersed From The Nuclear Speckles To The Cytoplasm |
| Alternative Antibody Names | Anti-Splicing Factor 3b Subunit 1 antibodyAnti-Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit antibodyAnti-Sf3b155 antibodyAnti-Spliceosome-Associated Protein 155 antibodyAnti-Sap 155 antibodyAnti-SF3B1 antibodyAnti-SAP155 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
