Anti-SETMAR antibody (350-400 aa) (STJ95625)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human SETMAR at the amino acid range 350-400
General Information
| Short Description | Rabbit polyclonal anti-Histone-lysine N-methyltransferase SETMAR (350-400 aa) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SETMAR |
| Gene ID | 6419 |
| Uniprot ID | SETMR_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human SETMAR at the amino acid range 350-400 |
| Immunogen Region | 350-400 aa |
| Specificity | SETMAR Polyclonal Antibody detects endogenous levels of SETMAR protein. |
Additional Info
| Post Translational Modifications | Methylated. Methylation regulates activity in DNA decatenation. Phosphorylated at Ser-508 by CHEK1 and dephosphorylated by protein phosphatase 2A/PP2A. Phosphorylation at Ser-508 is enhanced by DNA damage and promotes recruitment to damaged DNA. It stimulates DNA repair and impairs replication fork restart. |
| Function | Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Also has kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining. Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A. |
| Protein Name | Histone-Lysine N-Methyltransferase SetmarSet Domain And Mariner Transposase Fusion ProteinMetnase Includes - Histone-Lysine N-Methyltransferase - Transposon Hsmar1 Transposase |
| Database Links | |
| Cellular Localisation | NucleusChromosomeRecruited On Damaged Dna At Sites Of Double-Strand Breaks |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setmar antibodyAnti-Set Domain And Mariner Transposase Fusion Protein antibodyAnti-Metnase Includes - Histone-Lysine N-Methyltransferase - Transposon Hsmar1 Transposase antibodyAnti-SETMAR antibody |
Information sourced from Uniprot.org