Anti-SETDB1 antibody [5H6A12] (STJ98056)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ESET expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Histone-lysine N-methyltransferase SETDB1 for use in WB, IF and ELISA in Human, Mouse and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Mouse |
| Reactivity | Human/Mouse/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5H6A12 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SETDB1 |
| Gene ID | 9869 |
| Uniprot ID | SETB1_HUMAN |
| Immunogen | Purified recombinant fragment of human ESET expressed in E. Coli. |
| Specificity | ESET Monoclonal Antibody detects endogenous levels of ESET protein. |
Additional Info
| Post Translational Modifications | Degraded by the proteasome, shielded by interaction with ATF7IP. Monoubiquitinated at Lys-867 by E2 enzymes of the UBE2E family. The conjugated-Ub is protected from deubiquitination by the SET domain. Monoubiquitination at Lys-867 is required for catalytic activity, H3K9 methylation and endogenous retrovirus silencing. |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway. Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions. |
| Protein Name | Histone-Lysine N-Methyltransferase Setdb1Erg-Associated Protein With Set DomainEsetHistone H3-K9 Methyltransferase 4H3-K9-Hmtase 4Lysine N-Methyltransferase 1eSet Domain Bifurcated 1 |
| Database Links | Reactome: R-HSA-3214841Reactome: R-HSA-9843940Reactome: R-HSA-9843970 |
| Cellular Localisation | NucleusCytoplasmChromosomeAssociated With Non-Pericentromeric Regions Of ChromatinExcluded From Nucleoli And Islands Of Condensed Chromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setdb1 antibodyAnti-Erg-Associated Protein With Set Domain antibodyAnti-Eset antibodyAnti-Histone H3-K9 Methyltransferase 4 antibodyAnti-H3-K9-Hmtase 4 antibodyAnti-Lysine N-Methyltransferase 1e antibodyAnti-Set Domain Bifurcated 1 antibodyAnti-SETDB1 antibodyAnti-ESET antibodyAnti-KIAA0067 antibodyAnti-KMT1E antibody |
Information sourced from Uniprot.org