Western blot analysis of various lysates using Setd5 Rabbit pAb (STJ11106768) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Setd5 antibody (STJ11106768)

SKU:
STJ11106768
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Gene Symbol: Setd5
Gene ID: 72895
Uniprot ID: SETD5_MOUSE
Specificity: A synthetic peptide of human Setd5
Tissue Specificity Ubiquitously expressed.
Function Chromatin regulator required for brain development: acts as a regulator of RNA elongation rate, thereby regulating neural stem cell (NSC) proliferation and synaptic transmission. May act by mediating trimethylation of 'Lys-36' of histone H3 (H3K36me3), which is essential to allow on-time RNA elongation dynamics. Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. The relevance of histone methyltransferase activity is however subject to discussion.
Protein Name Histone-Lysine N-Methyltransferase Setd5
Set Domain-Containing Protein 5
Cellular Localisation Nucleus
Chromosome
Localizes To Active Transcribed Genes
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Setd5 antibody
Anti-Set Domain-Containing Protein 5 antibody
Anti-Setd5 antibody
Anti-Kiaa1757 antibody

Information sourced from Uniprot.org

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