Anti-SECISBP2 antibody (585-854) (STJ28819)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SECISBP2 (585-854) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SECISBP2 |
| Gene ID | 79048 |
| Uniprot ID | SEBP2_HUMAN |
| Immunogen Region | 585-854 |
| Immunogen Sequence | DELISTPSVEDKSEEPPGTE LQRDTEASHLAPNHTTFPKI HSRRFRDYCSQMLSKEVDAC VTDLLKELVRFQDRMYQKDP VKAKTKRRLVLGLREVLKHL KLKKLKCVIISPNCEKIQSK GGLDDTLHTIIDYACEQNIP FVFALNRKALGRSLNKAVPV SVVGIFSYDGAQDQFHKMVE LTVAARQAYKTMLENVQQEL VGEPRPQAPPSLPTQGPSCP AEDGPPALKEKEEPHYIEI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human SECISBP2 (NP_076982.3). |
Additional Info
| Tissue Specificity | Expressed at high levels in testis. |
| Function | mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. |
| Protein Name | Selenocysteine Insertion Sequence-Binding Protein 2Secis-Binding Protein 2 |
| Database Links | Reactome: R-HSA-2408557 |
| Cellular Localisation | Isoform 1: NucleusIsoform 2: Mitochondrion |
| Alternative Antibody Names | Anti-Selenocysteine Insertion Sequence-Binding Protein 2 antibodyAnti-Secis-Binding Protein 2 antibodyAnti-SECISBP2 antibodyAnti-SBP2 antibody |
Information sourced from Uniprot.org