Confocal imaging of HeLa cells usingย SDHA Rabbit monoclonal antibody (STJ11101431, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-SDHA antibody (550-650) [S1MR] (STJ11101431)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101431
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General Information

Short DescriptionRabbit monoclonal SDHA (550-650) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS1MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:6000
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSDHA
Gene ID6389
Uniprot IDSDHA_HUMAN
Immunogen Region550-650
Immunogen SequenceKTFDRGMVWNTDLVETLELQ NLMLCALQTIYGAEARKESR GAHAREDYKVRIDEYDYSKP IQGQQKKPFEEHWRKHTLSY VDVGTGKVTLEYRPVIDKTL N
SpecificityA synthetic peptide corresponding to a sequence within amino acids 550-650 of human SDHA (P31040).

Additional Info

Post Translational Modifications Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation. Acetylated. Deacetylated by SIRT3.
Function Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate. Can act as a tumor suppressor.
Protein Name Succinate Dehydrogenase Ubiquinone Flavoprotein Subunit - Mitochondrial
Flavoprotein Subunit Of Complex Ii
Fp
Malate Dehydrogenase Quinone Flavoprotein Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-71403
Reactome: R-HSA-9854311
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Succinate Dehydrogenase Ubiquinone Flavoprotein Subunit - Mitochondrial antibody
Anti-Flavoprotein Subunit Of Complex Ii antibody
Anti-Fp antibody
Anti-Malate Dehydrogenase Quinone Flavoprotein Subunit antibody
Anti-SDHA antibody
Anti-SDH2 antibody
Anti-SDHF antibody

Information sourced from Uniprot.org

Citations

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