• Immunofluorescence analysis of U2OS cells using SDHA Rabbit polyclonal antibody (STJ25461) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SDHA Rabbit polyclonal antibody (STJ25461) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SDHA Rabbit polyclonal antibody (STJ25461) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SDHA antibody (STJ25461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SDHA antibody (385-664) (STJ25461)

SKU:
STJ25461

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDHA
Gene ID: 6389
Uniprot ID: SDHA_HUMAN
Immunogen Region: 385-664
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).
Immunogen Sequence: ETAMIFAGVDVTKEPIPVLP TVHYNMGGIPTNYKGQVLRH VNGQDQIVPGLYACGEAACA SVHGANRLGANSLLDLVVFG RACALSIEESCRPGDKVPPI KPNAGEESVMNLDKLRFADG SIRTSELRLSMQKSMQNHAA VFRVGSVLQEGCGKISKLYG DLKHLKTFDRGMVWNTDLVE TLELQNLMLCALQTIYGAEA RKESRGAHAREDYKVRIDEY DYSKPIQGQQKKPFEEHWR
Post Translational Modifications Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation. Acetylated. Deacetylated by SIRT3.
Function Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate. Can act as a tumor suppressor.
Protein Name Succinate Dehydrogenase Ubiquinone Flavoprotein Subunit - Mitochondrial
Flavoprotein Subunit Of Complex Ii
Fp
Malate Dehydrogenase Quinone Flavoprotein Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-71403
Reactome: R-HSA-9854311
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Succinate Dehydrogenase Ubiquinone Flavoprotein Subunit - Mitochondrial antibody
Anti-Flavoprotein Subunit Of Complex Ii antibody
Anti-Fp antibody
Anti-Malate Dehydrogenase Quinone Flavoprotein Subunit antibody
Anti-SDHA antibody
Anti-SDH2 antibody
Anti-SDHF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance