• Confocal imaging of human tonsil using CD138/Syndecan-1 Rabbit monoclonal antibody (STJ11102010, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using CD138/Syndecan-1 Rabbit monoclonal antibody (STJ11102010) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from various cell lines, using Syndecan 1 Rabbit monoclonal antibody (STJ11102010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SDC1 antibody (23-254) [S0MR] (STJ11102010)

SKU:
STJ11102010

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Syndecan-1/CD138 (23-254) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDC1
Gene ID: 6382
Uniprot ID: SDC1_HUMAN
Immunogen Region: 23-254
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-254 of human Syndecan 1 (P18827).
Immunogen Sequence: QIVATNLPPEDQDGSGDDSD NFSGSGAGALQDITLSQQTP STWKDTQLLTAIPTSPEPTG LEATAASTSTLPAGEGPKEG EAVVLPEVEPGLTAREQEAT PRPRETTQLPTTHLASTTTA TTAQEPATSHPHRDMQPGHH ETSTPAGPSQADLHTPHTED GGPSATERAAEDGASSQLPA AEGSGEQDFTFETSGENTAV VAVEPDRRNQSPVDQGATGA SQGLLDRKEVLG
Tissue Specificity Detected in placenta (at protein level). Detected in fibroblasts (at protein level).
Post Translational Modifications Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Function Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway.
Protein Name Syndecan-1
Synd1
Cd Antigen Cd138
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-202733
Reactome: R-HSA-3000170
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-4420332
Reactome: R-HSA-449836
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Secreted
Extracellular Exosome
Shedding Of The Ectodomain Produces A Soluble Form
Alternative Antibody Names Anti-Syndecan-1 antibody
Anti-Synd1 antibody
Anti-Cd Antigen Cd138 antibody
Anti-SDC1 antibody
Anti-SDC antibody

Information sourced from Uniprot.org

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