| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SCRIB (1100-1400) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SCRIB |
| Gene ID: |
23513 |
| Uniprot ID: |
SCRIB_HUMAN |
| Immunogen Region: |
1100-1400 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1400 of human SCRIB (NP_874365.3). |
| Immunogen Sequence: |
ELCIQKAPGERLGISIRGGA RGHAGNPRDPTDEGIFISKV SPTGAAGRDGRLRVGLRLLE VNQQSLLGLTHGEAVQLLRS VGDTLTVLVCDGFEASTDAA LEVSPGVIANPFAAGIGHRN SLESISSIDRELSPEGPGKE KELPGQTLHWGPEATEAAGR GLQPLKLDYRALAAVPSAGS VQRVPSGAAGGKMAESPCSP SGQQPPSPPSPDELPANVKQ AYRAFAAVPTSHPPEDAPA |
| Tissue Specificity | Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). |
| Post Translational Modifications | Ubiquitinated.targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded. Palmitoylated. Could be depalmitoylated by LYPLA1 and/or LYPLA2. Palmitoylation of SCRIB by ZDHHC7 is required for its localization to cell-cell junctions, function in the establishement of epithelial cell polarity and the regulation of downstream signaling pathways important for epithelial cell differentiation. |
| Function | Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization. Via its interaction with CRTAM, required for the late phase polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG and IL22 production. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting of synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. |
| Protein Name | Protein Scribble HomologScribbleHscribProtein Lap4 |
| Database Links | Reactome: R-HSA-4608870Reactome: R-HSA-9013148Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9696264Reactome: R-HSA-9696270 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCell JunctionAdherens JunctionCell ProjectionLamellipodiumCytoplasmPostsynapsePresynapseTargeting To Cell-Cell Junctions Which Is Cdh1-Dependent Is Required For The Pro-Apoptotic ActivityIn A Subset Of Cd4+ T-CellsColocalizes With Crtam At The Immunological Synapse During The Late Phase Of T-Cell Activation |
| Alternative Antibody Names | Anti-Protein Scribble Homolog antibodyAnti-Scribble antibodyAnti-Hscrib antibodyAnti-Protein Lap4 antibodyAnti-SCRIB antibodyAnti-CRIB1 antibodyAnti-KIAA0147 antibodyAnti-LAP4 antibodyAnti-SCRB1 antibodyAnti-VARTUL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
