• Western blot analysis of extracts of various cell lines, using SCFD1 antibody (STJ111440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using SCFD1 Rabbit polyclonal antibody (STJ111440) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using SCFD1 rabbit polyclonal antibody (STJ111440) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SCFD1 Rabbit polyclonal antibody (STJ111440) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using SCFD1 rabbit polyclonal antibody (STJ111440) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using SCFD1 Rabbit polyclonal antibody (STJ111440) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat lung using SCFD1 rabbit polyclonal antibody (STJ111440) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using SCFD1 antibody (STJ111440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SCFD1 antibody (373-642) (STJ111440)

SKU:
STJ111440

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCFD1 (373-642) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCFD1
Gene ID: 23256
Uniprot ID: SCFD1_HUMAN
Immunogen Region: 373-642
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 373-642 of human SCFD1 (NP_057190.2).
Immunogen Sequence: SMLSDNTAKLTSAVSSLPEL LEKKRLIDLHTNVATAVLEH IKARKLDVYFEYEEKIMSKT TLDKSLLDIISDPDAGTPED KMRLFLIYYISTQQAPSEAD LEQYKKALTDAGCNLNPLQY IKQWKAFTKMASAPASYGST TTKPMGLLSRVMNTGSQFVM EGVKNLVLKQQNLPVTRILD NLMEMKSNPETDDYRYFDPK MLRGNDSSVPRNKNPFQEAI VFVVGGGNYIEYQNLVDYI
Function Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.
Protein Name Sec1 Family Domain-Containing Protein 1
Sly1 Homolog
Sly1p
Syntaxin-Binding Protein 1-Like 2
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-8980692
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Golgi Apparatus
Golgi Stack Membrane
Alternative Antibody Names Anti-Sec1 Family Domain-Containing Protein 1 antibody
Anti-Sly1 Homolog antibody
Anti-Sly1p antibody
Anti-Syntaxin-Binding Protein 1-Like 2 antibody
Anti-SCFD1 antibody
Anti-C14orf163 antibody
Anti-KIAA0917 antibody
Anti-STXBP1L2 antibody
Anti-FKSG23 antibody

Information sourced from Uniprot.org

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