| Short Description : | Rabbit polyclonal SCAF4 (170-248) antibody for use in IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SCAF4 |
| Gene ID: | 57466 |
| Uniprot ID: | SCAF4_HUMAN |
| Immunogen Region: | 170-248 |
| Immunogen Sequence: | TPNSVPAVPQLPSSDAFAAV AQLFQTTQGQQLQQILQTFQ QPPKPQSPALDNAVMAQVQA ITAQLKTTPTQPSEQKAAF |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-248 of human SCAF4 (NP_065757.1). |
| Function | Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF8, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF8, also acts as a suppressor of transcriptional readthrough. |
| Protein Name | Sr-Related And Ctd-Associated Factor 4Ctd-Binding Sr-Like Protein Ra4Splicing Factor - Arginine/Serine-Rich 15 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Sr-Related And Ctd-Associated Factor 4 antibodyAnti-Ctd-Binding Sr-Like Protein Ra4 antibodyAnti-Splicing Factor - Arginine/Serine-Rich 15 antibodyAnti-SCAF4 antibodyAnti-KIAA1172 antibodyAnti-SFRS15 antibody |
Information sourced from Uniprot.org
