Western blot analysis of lysates from Mouse placenta, using SAT1 Rabbit polyclonal antibody (STJ26208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SAT1 antibody (1-171) (STJ26208)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ26208
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General Information

Short DescriptionRabbit polyclonal SAT1 (1-171) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSAT1
Gene ID6303
Uniprot IDSAT1_HUMAN
Immunogen Region1-171
Immunogen SequenceMAKFVIRPATAADCSDILRL IKELAKYEYMEEQVILTEKD LLEDGFGEHPFYHCLVAEVP KEHWTPEGHSIVGFAMYYFT YDPWIGKLLYLEDFFVMSDY RGFGIGSEILKNLSQVAMRC RCSSMHFLVAEWNEPSINFY KRRGASDLSSEEGWRLFKID KEYLLKMATEE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-171 of human SAT1 (NP_002961.1).

Additional Info

Function Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Also acts on 1,3-diaminopropane and 1,5-diaminopentane.
Protein Name Diamine Acetyltransferase 1
Polyamine N-Acetyltransferase 1
Putrescine Acetyltransferase
Spermidine/Spermine N(1-Acetyltransferase 1
Ssat
Ssat-1
Database Links Reactome: R-HSA-351200
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Diamine Acetyltransferase 1 antibody
Anti-Polyamine N-Acetyltransferase 1 antibody
Anti-Putrescine Acetyltransferase antibody
Anti-Spermidine/Spermine N(1-Acetyltransferase 1 antibody
Anti-Ssat antibody
Anti-Ssat-1 antibody
Anti-SAT1 antibody
Anti-SAT antibody

Information sourced from Uniprot.org

Citations

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