• Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP8 Protein, using SARS-CoV-2 NSP8 Rabbit polyclonal antibody (STJ11103224) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SARS-CoV-2-NSP8 antibody (1-198) (STJ11103224)

SKU:
STJ11103224

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Host: Rabbit
Applications: WB
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-NSP8 (1-198) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-198
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of coronavirus NSP8 (YP_009742615.1).
Immunogen Sequence: AIASEFSSLPSYAAFATAQE AYEQAVANGDSEVVLKKLKK SLNVAKSEFDRDAAMQRKLE KMADQAMTQMYKQARSEDKR AKVTSAMQTMLFTMLRKLDN DALNNIINNARDGCVPLNII PLTTAAKLMVVIPDYNTYKN TCDGTTFTYASALWEIQQVV DADSKIVQLSEISMDNSPNL AWPLIVTALRANSAVKLQ
Background This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

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