• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 NSP4 using SARS-CoV-2 NSP4 Rabbit polyclonal antibody (STJ11103253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 20  Mu g/10 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SARS-CoV-2-NSP4 antibody (400-500) (STJ11103253)

SKU:
STJ11103253

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Host: Rabbit
Applications: WB
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-NSP4 (400-500) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 400-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of coronavirus NSP4 (YP_009725300.1).
Immunogen Sequence: RRVVFNGVSFSTFEEAALCT FLLNKEMYLKLRSDVLLPLT QYNRYLALYNKYKYFSGAMD TTSYREAACCHLAKALNDFS NSGSDVLYQPPQTSITSAVL Q
Background Enables RNA stem-loop binding activity. Involved in IRES-dependent viral translational initiation; nuclear-transcribed mRNA catabolic process, no-go decay; and stress granule assembly. Located in Golgi apparatus; cytosol; and plasma membrane. Part of CRD-mediated mRNA stability complex.

Information sourced from Uniprot.org

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